The Chronic Crohns Campaign UK. ( TCCC.UK ).
The Chronic Crohns Campaign UK - Why We Are Here !!!
TCCC.UK & TNHC.UK Campaign Literature.
The Chronic Crohns Campaign UK - Other TCCC.UK Contacts
TCCC.UK - Funds To Kings College London.
TCCC.UK - Action Medical Research details.........
TCCC.UK Campaign News ,Updates and Success !!!
The Chronic Crohns Campaign UK- The Breaking News.
TCCC.UK &The 3 Natural Alternatives In Sarah's Story
The New DNA Crohns Vaccine @ Kings College London.
TCCC.UK & Aloe Vera - Nature's Silent Healer. Books
TCCC.UK Raising Awareness - Press Coverage 1996 to 2009.
TCCC.UK - More Breaking News On MAP !!!!!!!!!!!!!!!!!!!!!!
Sarah's Crohns Success Story From 1989 to 2006.
Sarah's Crohns Success Story From 2001 to 2006.
Over 90 Degrees Still Does Not Kill Map Bacteria In Milk.
Tim Page - My Side Of The Story In All This Campaigning.
How To Order Aloe Vera, As We Do, At A Lower Price In The UK.
How To Get A Glyconutrient Powder Here In The UK
"Give Us A Quid Or Two" -The DNA Crohns Vaccine Appeal
PARA'S Medical Advisory Council
PARA'S Scientific Advisory Council.
A Message To Internet Hackers / Spammers !!
In Loving Memory Of Hadge Elliott 1956 To 2005.
The New DNA CrohnsVaccine Summary 2006.
TCCC.UK Consultation with DEFRA in 2002 & 2004.
The Chronic Crohns Campaign UK Press Info 1996 to 2009.
The Orkney Islands Crohns & U.C. N A C Group
Birmingham Contact For TCCC.UK - Stuart Morris IIHHT ICHT dip
MMR = Crohns or Autism ?
How To Get Aloe Vera & Other Aloe Vera Items Outside UK.
Crohns & Contraception.
More On MAP - The 2 AntibioticsTreatment By Prof JHT.
Other Interesting Natural Health Documents.
More On The Bacteria MAP - 2
Scottish News On Crohns
More On Fluoride
TCCC.UK & The BBC
Baby Milk - The FSA Update December 2006.
Prof John Hermon Taylor's New Update In March 2008.
Christmas 2009 & New Year 2010.
Crohns Sufferers - Some National Figures @ 2006
Crohns Disease By Eye Online
Intracellular Pathogen Group/St George's University London.
The Italian Crohns Campaign Connections.
Dr Ira Shafron M.D
For Jewellery Findings & Supplies In Tunbridge Wells, Kent.
A Friendly Ileostomy Councillor For Contact & Advice.
A Memorial Page To My Grandad Who Inspired Me.
Crohns & Food Intolerances & Illness.
TCCC.UK Petition To The UK Prime Minister + Another Petition
DrugWatch Check Them Out...
Crohns Research or IBD or IBS Stories In The News
Prof John Hermon -Taylor On You Tube & TV 2008..
Support Our New DNA Crohns Vaccine Fundraisers.
Further Water And Milk Press Coverage.
Can MAP Cause Ulcerated Colitis, As It Does Crohns..
TCCC.UK - Why We Are Here In 2008, To Help & Advise You.
TCCC.UK - Announcements In 2008.
Useful Addresses For Complaints On The NHS.
Hannah's Real Story - from 2006 to 2011....Ongoing.
Prof John Hermon -Taylor Updates In 2009.
Scientific Papers On IBDs.
New Crohns Book of Tim Page Crusader.
Need A Good Crohns Doctor ?
Prof John Hermon Taylor In Washington USA YouTube Links
New Crohns Support Group On Facebook
Tim Page - TCCC.UK On FaceBook & More....
Crohns Disease Contact The Chronic Crohns Campaign UK
TCCC.UK - Fundraising Events 2009
" MAP Doomsday " By Prof John Hermon Taylor - August 2
The Gift by Stuart Morris @ WonderfullyWell
New Youtube IBD Videos on Crohns & MAP.
TCCC.UK - Crohns Support Groups On Facebook.
Mycobacterium sub species Avium Paratuberculosis ( MAP )
Prof John Hermon Taylor -2010, 2011, 2012 - Updates.
Alternatives & Immunesuppressants - Working Together.
House Of Lords Debate On Crohns Disease 19th June 2000.
Sarah's Success Story Update In 2012
A Memorial Page To Cathy Lynn Bray -10/09/67 to 24/02/2012
28,000 TB Infected Cattle Enter Human Food chain
What's News From TCCC.UK In 2013
Tim Page - Founder of TCCC.UK in 2013.
Contact Information for The Chronic Crohns Campaign UK
Links for The Chronic Crohns Campaign UK
Do You Find This Crohns Site Useful ?
PARA'S Scientific Advisory Council.
Dr Tim Bull.
Dr. Timothy Bull.
Senior Research Fellow
St. George's University Hospital Medical School,
Tim Bull has a Masters Degree in Bacteriology, a further Masters in Virology, and a PhD (Thesis : Mycobacterial phylogenetics) from The University of London, UK. His current position is Senior Research Fellow in Dept. Cardiovascular Medicine - Surgery, St George's Hospital Medical School, London, UK. His field of research involves the investigation of molecular mechanisms associated with the virulence of bacterial infection in animals and humans, with particular interest in the relationship to pathogenicity, phenotype and virulence of transposable Insertion Elements and unique mycobacterial associated proteins. His previous studies have identified and described new mycobacterial species (Mycobacterium celatum and Mycobacterium mageritense) and novel mycobacterial insertion sequences (IS1407, IS1511, IS1512, IS1612, IS1613). He has developed sensitive methods that have enabled accurate and rapid detection of M. avium subsp. paratuberculosis (MAP) in gut tissue from animals with Johne's disease and humans with Crohn's disease. Further work has developed novel DNA based systems for the rapid typing and effective epidemiological study of MAP infection in animals and humans. Recent studies include the characterisation of genes in MAP with associations to pathogenicity and mapping of IS900 insertions in the MAP genome. He is also involved in work constructing specific mycobacterial gene knockouts for use in differential expression analysis of the MAP transcriptome and generation of attenuated MAP strains for possible use in animal vaccination. Current work involves the design and development of anti-MAP vaccines based on MAP specific cell wall antigens using a recombinant vaccinia viral delivery system. This is aimed at therapeutic use, acting to stimulate immune recognition and elimination of chronic MAP infection.
Dr Salek A.Naser.
Dr Salec A Naser.
Dr. Saleh A. Naser.
Department of Molecular Biology and Microbiology
University of Central Florida. U.S.A.
WWW : http://pegasus.cc.ucf.edu/~nasers
Dr. Naser is a microbiologist and Assistant Professor at the University of Central Florida. He has a particular interest in research into the pathogenesis of Crohn's disease and drug discovery for treatment of multiple drug resistant pathogens such as Mycobacterium tuberculosis and Staphylococcus aureus. He teaches Advanced Clinical Chemistry and Infectious Process. Dr. Naser is Co-author of a Clinical Chemistry Laboratory Manual.
His twelve published papers all relate to some aspect of mycobacteria. One of his recently published papers describes the discovery of MAP in the breast milk of lactating Crohn's patients. (S. A. Naser, I. Shafran, D. Swartz. Isolation of Mycobacterium avium subsp. paratuberculosis from Breast Milk of Crohn's Disease Patients.)
Dr John Belisle.
Dr John Belisle.
Dr. John T. Belisle.
College of Veterinary Medicine & Biomedical Sciences,
Colorado State University,
Fort Collins, CO. U.S.A.
WWW : http://www.cvmbs.colostate.edu/microbiology/belisle.htm
Dr. Belisle: "My research focuses on two areas of mycobacterial genetics and physiology. (1) The cellular envelope of Mycobacterium spp. is comprised of several unique glycolipids, polysaccharides and long chain fatty acids. We are currently working to identify the genes encoding the biosynthesis of these structures so that their roles in pathogenesis can be evaluated and to provide tools for the rational design of new antimycobacterial drugs. This work is being conducted in collaboration with Drs. Patrick Brennan, Julie Inamine, Gurdyal Besra, Delphi Chatterjee and Mike McNeil. (2) Protein antigens of M. tuberculosis are the primary target of the protective immune response against this organism and the contributions of these molecules to pathogenesis are now being realized. We are currently focusing our efforts on defining unique glycoproteins of M. tuberculosis and the implication of these post-translational modifications on immunogenicity and bacterial physiology. Additionally, unique proteins present only in clinical isolates of M. tuberculosis are being evaluated for their immunological characteristics and physiological function.
Dr Roderick Chiodini
Dr Roderick Chiodini.
Dr. Rodrick J. Chiodini.
WWW : http://www.paratuberculosis.org/members/chiodini.htm
Dr. Chiodini received his Ph.D. in Bacteriology from the University of Connecticut with a specialty in Mycobacteriology. His primary research focus and Ph.D. thesis subject was paratuberculosis in cattle. In the late 1970's and early 1980's, Dr. Chiodini made the transition from animal disease to human disease and began to investigate the relationship between mycobacteria and Crohn's Disease in humans. His early pioneering efforts provided the first documentation and evidence that M. paratuberculosis was associated with Crohn's Disease, and caused the suspicion to become a credible theory of the cause of Crohn's Disease. Throughout most of the 1980's, Dr. Chiodini was in the forefront of investigation related to mycobacteria and Crohn's Disease, and led the research community into this controversial subject. Even today, much research relating to M. paratuberculosis in Crohn's Disease is derived from the infrastructure and backbone created by Dr. Chiodini. After over 20 years of effort in this area, no-one understands the implications or politics of Crohn's Disease and mycobacteria better than Dr. Chiodini. PARA is fortunate to have a scientist on its advisory council who provides the unique perspective of having been present on the ground floor of this controversial issue.
Dr. Chiodini was the co-founder and past-president of the International Association for Paratuberculosis, and has published over one hundred articles related to M. paratuberculosis and Crohn's Disease.
Dr Michael T Collins.
Dr Michael T Collins.
Dr. Michael T. Collins.
Professor of Microbiology
School of Veterinary Medicine.
University of Wisconsin at Madison, U.S.A.
WWW : http://www.johnes.org
As a veterinarian and a microbiologist, Dr. Collins approaches study of MAP with two primary interests: 1) diagnosis and control of the infection in animals, and 2) understanding the biology of this unusual pathogen. His research publications span a wide variety of subjects, from cost-benefit analysis of a test and slaughter control program in dairy herds to laboratory studies on thermal tolerance of the organism to help understand survival characteristics of this bacterium in pasteurized foods.
Most recently, Dr. Collins has turned his interest in paratuberculosis diagnostics toward the human species. In addition to his teaching and research as part of his job as Professor at the University of Wisconsin School of Veterinary Medicine, Dr. Collins serves on the National (USA) Johne's Working Group, (NJWG) and is President of the International Association for Paratuberculosis.
Dr Fouad A K EL Zaatari.
|Dr Fouad A K EL Zaatari.|
Dr Fouad A K El - Zaatari,
VAMC and Baylor College of Medicine,
Dept of Medicine/GI Section
2002.Holcombe Blvd (111D)
Texas. 77030, USA.
Tel No : (713) 794-7213.
Fax No : (713) 790-1040.
Research / Work.
Expertise : Molecular-microbiology, Molecular-immunology, and Molecular-genetics, Diagnostics and Research.
Active In : Molecular Biology/Microbiology/Crohn's Disease.
Description : Molecular characterization of Mycobacterium paratuberculosis and it's role in pathogenesis of Crohn's disease and sarcoidosis in human and Johne's disease in animals.
Dr. Fouad El-Zaatari - Investigations of the Humoral Immune Response in Crohn’s Pathogenesis
Dr. El-Zaatari discussed the status of diagnosis of Map infection by serology. Bacterial antigens which react specifically with the sera of CD patients and which can serve as diagnostic for Map have been looked for for many years. Dr. El-Zaatari said the difficulty in identifying useful antigens could be attributed to the relative impurity of the antigens and mixtures of antigens studied and found to be non-specific. This is not surprising when one considers the many other mycobacterial species humans can be exposed to. It is necessary to work with purified antigens or preferably epitopes from these antigens in order to make diagnostic tests more specific.
Dr. El-Zaatari has identified 24 Map antigens by immunological screening of expression libraries with serum from Crohn’s patients. These antigens ranged from 18 to 80 Kda in size. Two of these, a 35 Kda and a 36 Kda antigen, show promise as being diagnostic for CD.
P35 detected 100% of animals with clinically positive Johne’s disease, 75% of sub-clinical animals, and was non-reactive in 15 normal animals. In sera from humans with CD, P35 detected 75% as positive; 10% of ulcerative colitis; 14% of controls; 25% of tuberculosis; and 0% of leprosy cases. This appears to be an interesting antigen that may be specific at the genus and species levels. It may be useful for detecting clinical and subclinical Johne’s disease and CD. An epitope from this antigen is being pursued as a diagnostic.
P36 was reactive with 89% of the CD sera. However, it seemed less discriminating than P35 as 75% (6 of 8) TB sera were positive; 100% (10 of 10) leprosy sera; and 100% (7 of 7) sarcoidosis sera were positive. Only 15% of ulcerative colitis (N=27), and 7% of non-IBD sera (N=15) were positive. Animals with Johne’s did not react with this molecule. This antigen reacted with antibody produced against eight different mycobacterial species. When P35 and P36 are used together, the specificity increased to 98%. The sensitivity of the pair is close to that for P35. The positive predictive value also is 98% and negative predictive value is 76% for the pair. Identification of reactive epitopes of these two antigens may make them even better diagnostics.
Question : Is it possible that these antigens are lipoproteins?
Answer :Yes. P35 is expressed on the surface and is secreted according to the Belgium group. I have no idea what their function is. They are useful as diagnostics. We just have to work more and identify more specific epitopes.
Dr Norman Pace.
Dr Norman Pace.
Dr. Norman R. Pace.
Director, Pace Laboratory
Department of Molecular, Cellular & Developmental Biology
University of Colorado at Boulder, U.S.A.
WWW : http://mcdb.colorado.edu/faculty/pace99.html
Current research interests revolve about the synthesis, structure and function of RNA; and the application of molecular biological tools to problems in environmental microbial biology.
A main effort of the laboratory is on the study of RNase P, an RNA processing enzyme responsible for removing the 5' termini of tRNA precursors during their maturation. This is a particularly interesting enzyme because the catalytic element is itself an RNA; RNase P is a ribozyme. Studies underway include analyses of the structure of the RNase P RNA, the nature of its interaction with tRNA precursors and other substrates, and the mechanism of the RNA-catalyzed reaction.
A second emphasis is on developing methods for analyzing phylogenetic and quantitative aspects of natural microbial populations without the necessity for laboratory cultivation. The methods rely on cloning and sequencing ribosomal RNA (rRNA) genes for phylogenetic analyses, and the use of rRNA-based hybridization probes for various studies of microbial ecosystems. Investigations are broadly directed, for instance toward high-temperature environments, unusual symbioses, environmental bioremediation and selected human diseases.
Paratuberculosis Awareness & Research Association.
Paratuberculosis Awareness & Research Association.
Mailing Address :
P.O. Box 16219.
Temple Terrace, Florida 33687-6219.
Visit PARA's website at http://www.crohns.org
for extensive information about MAP and Crohn's disease.
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